Figure 4. The dg2 Gene Is Involved in Surviving Prolonged Hypoxia.

(A) The dg2 gene is required in embryos for survival during prolonged hypoxia. Stage 12 for^R and for^s embryos were subjected to hypoxia for either 2, 4, 6, or 12 hr and then removed and allowed to develop at 25°C on grape agar plates for 30 hr. The percent viability was determined by the number of larvae that hatched. Viability of for^R embryos: 0 hr, 96%; 2 hr, 92% ± 2.8%; 4 hr, 90% ± 2.9%; 6 hr, 93% ± 5%; 12 hr, 97% ± 2.9%. Viability of for^s embryos: 0 hr, 89%; 2 hr, 89% ± 8.7%; 4 hr, 82% ± 8.6%; 12 hr, 18% ± 5.8%. Percentages are the average of three experiments.

(B) The dg2 gene is required in larvae for survival during prolonged hypoxia. Early third instar for^R and for^s larvae were subjected to hypoxia for 6 hr, after which they were removed and allowed to develop on grape agar plates containing yeast. The percent viability was determined by the number of flies that eclosed. Viability of for^R larvae, 83% ± 11.1%; for^s larvae, 24% ± 4.0%. Percentages are the average of three experiments.