Skip to main content
. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Cell. 1999 Jul 9;98(1):105–114. doi: 10.1016/S0092-8674(00)80610-8

Figure 6. NO and PKG Promote Tracheal Branching in Larvae.

Figure 6

(A and B) fors larvae are deficient in tertiary tracheal branching. Anterior midguts immediately posterior to the proventriculus were dissected from early third instar forR (A) and fors (B) larvae and mounted in PBS with 5% Ficoll. Arrows indicate tertiary branches. Scale bar is 50 μm.

(C and D) Ectopic expression of iNOS increases tertiary tracheal branching. Early second instar Sevelen (C) and hs-iNOS (D) larvae were subjected to 30 min of heat shock at 37°C and then allowed to age for 12 hr followed by another 30 min heat shock. Twelve hours later, third instar larvae midguts were dissected and mounted as described in (A) and (B). Arrows indicate tertiary branches.

(E and F) Inhibition of NOS blocks tertiary tracheal branching. Wild-type larvae were fed from hatching either 0.5 M of D-NAME (E) or L-NAME (F). Midguts from early third instar larvae were dissected and mounted as described in (A and B). Arrows indicate tertiary branching.