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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: J Immunol. 2009 Jun 19;183(2):792–796. doi: 10.4049/jimmunol.0900173

Figure 1.

Figure 1

TNF-α induces caspase-1 activation in response to ATP and silica in the absence of microbial stimulation. A, BMDM were stimulated for 6 hrs with TNF (100 ng/ml), CD40L (10μg/ml), agonistic anti-Fas antibody (10μg/ml), IFN-γ (100 U/ml) or LPS (10 ng/ml). When indicated ATP (5 mM) was added for the last 30 minutes B, BMDM were stimulated for 6 hrs with PMA (100 ng/ml), RANKL (1 μg/ml), IL-1α (10 ng/ml), IL-1β (10 ng/ml). When indicated ATP (5 mM) was added for the last 30 minutes. C, BMDM were stimulated with TNF-α and then stimulated with ATP, silica or infected with S. Typhimurium (Salm). (A–C) Extracts were prepared from cell and culture supernatants and immunoblotted with caspase-1 antibody. Arrows denote procaspase-1 (p45) and its processed p20 subunit. Results are representative of three separate experiments.