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. Author manuscript; available in PMC: 2010 Sep 8.
Published in final edited form as: Cancer Cell. 2009 Sep 8;16(3):195–207. doi: 10.1016/j.ccr.2009.08.010

Figure 1. ErbB2 and 14-3-3ζ co-overexpression is associated with increased invasion potential in an in vitro DCIS model.

Figure 1

(A) Establishment of MCF10A stable cell lines overexpressing ErbB2 alone, 14-3-3ζ alone or both ErbB2 and 14-3-3ζ. Multiple stable clones were established for each sublines, and most experiments were repeated with different clones to rule out clonal effects. Immunoblot analysis of one representative of each subline for the indicated proteins is shown.

(B) Co-overexpression of ErbB2 and 14-3-3ζ led to anchorage-independent growth of MCF10A cells in soft agar assay.

(C) Distinct acinar structures of MCF10A sublines in 3D culture. Top: Phase-contrast images of acinar structures (19 days); bar: 200 μm. Inset: Amplified view of individual 10A.ErbB2.ζ cell invading into surrounding matrigel; bar: 100 μm.

(D) Loss of basement membrane integrity in 10A.ErbB2.ζ acini. MCF10A sublines were cultured in 3D matrigel for 28 days and then stained for laminin V (red) and DAPI (blue). Representative images were shown. Bar: 100 μm.