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. Author manuscript; available in PMC: 2010 Sep 8.
Published in final edited form as: Cancer Cell. 2009 Sep 8;16(3):195–207. doi: 10.1016/j.ccr.2009.08.010

Figure 6. Co-overexpression of ErbB2 and 14-3-3ζ promotes invasion and metastasis.

Figure 6

(A) 140 DCIS (top) and 107 IBC (bottom) samples were subjected to IHC staining for TβRI and 14-3-3ζ, of which 138 DCIS and 100 IBC cases were satisfactory for analysis. Chi-square test indicated a correlation between TβRI and 14-3-3ζ expression in both cohorts, P<0.05.

(B) Representative IHC staining for ErbB2, 14-3-3ζ, TβRI, E-cadherin, and N-cadherin in a DCIS case with microinvasion and a low-grade DCIS. Bar: 50 μm.

(C) 14-3-3ζ overexpression in TM15 cells increased lung metastasis. TM15 cells were stably transfected with HA-14-3-3ζ or empty vector. TM15.Vec and TM15.14-3-3ζ cells were injected into mammary fat pad of nude mice (four mice each group). When the primary tumors reached the size of 150 mm3, mice were sacrificed and their lungs underwent histological analysis to measure metastasis, P<0.05. Error bar indicates SEM. Experiment was repeated twice and similar results were observed.

(D) Co-overexpression of ErbB2 and 14-3-3ζ in IBC correlated with higher rates of death and disease recurrence. Tumor samples from 107 breast cancer patients were IHC-stained for ErbB2 and 14-3-3ζ, Kaplan-Meier plots of overall survival (left) and disease-free survival (right) according to ErbB2 and 14-3-3ζ expression are shown. P<0.05 (log-rank test).