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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: J Biol Chem. 2000 Oct 27;276(3):1930–1937. doi: 10.1074/jbc.M003911200

Fig. 4. NO treatment arrests the syncytial nuclear division in metaphase.

Fig. 4

His-GFP syncytial embryos were used to monitor the effect of NO treatment on the progression of the nuclear divisions in the absence of hypoxia in real time (see Fig. 3). When the nuclei were in prophase, as indicated by condensed chromatin (A), the embryos were treated with 10 mm SNAP, an NO donor, in saline solution. Nuclei progressed normally into metaphase (B) but remained blocked in metaphase with hypercondensed DNA (C) for the duration of the 10 min treatment. The embryos were then washed with saline solution to remove the NO-donor. Nuclei exhibited a 3-min delay and then exited mitosis normally. Anaphase was synchronous and with normal morphology and timing after the wash (D). The arrows follow a single nucleus through the treatment. The scale bar in D corresponds to 10 μm.