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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: J Mol Biol. 2009 Apr 21;389(3):595–605. doi: 10.1016/j.jmb.2009.04.030

Fig 1. Repression of transcription factor enhanced basal MMTV activity by LeTx.

Fig 1

Cos7 cells plated in 24-well plates were transfected with 100 ng pGL3-LTR-luc, 15 ng phRL-TK, in the absence or presence of indicated amounts of expression plasmids for specific transcription factors using FuGENE6. After 24 h incubation in media with or without LeTx (10 ng/ml LF + 500 ng/ml PA), the cells were lysed, and luciferase assayed. The effects of 20 or 40 ng/well of the transcription factors HNF3 (a), Oct1 (b), c-Jun (c), or a combination of c-Jun and c-Fos, each at 10 ng/well or 20 ng/well (d) on the transcriptional activity of the MMTV promoter in the presence (closed bars) or absence (open bars) of LeTx are shown. Each treatment was performed in triplicates, means and standard deviations from 3 independent experiments are shown. Two-way ANOVA followed by Bonferroni post-hoc test was performed (*, p = 0.01–0.05; * *, p = 0.001–0.01; ***, p ≤0.001).