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. Author manuscript; available in PMC: 2010 Jun 12.
Published in final edited form as: J Mol Biol. 2009 Apr 21;389(3):595–605. doi: 10.1016/j.jmb.2009.04.030

Fig 2. LeTx repression of PMA-induced AP-1 activation of the MMTV promoter.

Fig 2

Cos7 cells plated in 24-well plates were transfected with 100 ng pGL3-LTR-luc, 15 ng phRL-TK, 10 ng c-Jun and 10 ng c-Fos expression plasmids using FuGENE6. After 24 h incubation in media with vehicle only, 1 nM or 10 nM PMA in the presence (solid bars) or absence (open bars) of LeTx (10 ng/ml LF + 500 ng/ml PA), cells were lysed, and the luciferase assayed. Each treatment was performed in triplicates, means and standard deviations of the relative luciferase activities (vehicle only was normalized to 1). 2 separate experiments are shown. A two-way ANOVA followed by Bonferroni post-hoc test was performed (*, p = 0.01–0.05; **, p = 0.001–0.01; ***, p ≤0.001).