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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Free Radic Biol Med. 2007 May 16;43(4):568–580. doi: 10.1016/j.freeradbiomed.2007.05.009

FIGURE 3. Effect of GSH on curcumin-mediated suppression of TNF-induced AP-1 activity.

FIGURE 3

A, Curcumin suppressed TNF-induced AP-1 activity completely and GSH significantly inhibited the effect of curcumin. KBM-5 cells were incubated with indicated concentrations of GSH for 2 h, treated with 50 μM curcumin for 4 h. Then cells were activated with 0.1 nM of TNF for 30 min, and then subjected to EMSA to assay for AP-1 activation. B, The specificity of binding was examined by competition with unlabeled oligonucleotide. Nuclear extracts from untreated or TNF-treated cells were incubated with the cold unlabeled AP-1 oligo-probe then assayed for AP-1 activation by EMSA.