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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Clin Chem. 2009 Feb 20;55(4):748–756. doi: 10.1373/clinchem.2008.113381

Fig. 1. Outline of COLD-PCR/TaqMan genotyping.

Fig. 1

COLD-PCR/TaqMan genotyping is a 2-step amplification that uses denaturation at a Tc and annealing/extension at a single temperature. A TaqMan probe with a centrally located nucleotide matching the mutation is used for realtime detection. WT, wild type; MUT, mutant.