Fig. 2. Comparison of regular PCR/TaqMan genotyping with COLD-PCR/TaqMan genotyping for the T790M C>T mutation in EGFR exon 20.
Serial dilutions into wild-type DNA of DNA from the H1975 cell line containing the T790M mutation encoded by EGFR exon 20 were screened for the T790M mutation with regular PCR/TaqMan genotyping (A) and with COLD-PCR/TaqMan genotyping (MGB probe) (B).