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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: Clin Chem. 2009 Feb 20;55(4):748–756. doi: 10.1373/clinchem.2008.113381

Fig. 6. Two-round COLD-PCR/TaqMan genotyping of the T790M mutation.

Fig. 6

DNA from the H1975 cell line was serially diluted into human male DNA. Duplicate samples containing 0.1% and 0.05% T790M mutant and 11 replicates of reference wild-type (WT) DNA were tested with COLD-PCR/TaqMan genotyping. (A), Single-round COLD-PCR/TaqMan genotyping of the T790M mutation (104-bp amplicon). (B), Second-round (nested) COLD-PCR/TaqMan genotyping of the T790M mutation (67-bp amplicon).