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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Science. 2009 Sep 25;325(5948):1700–1704. doi: 10.1126/science.1176221

Figure 3.

Figure 3

Blinding antennal clocks alters their timing. (A) Irradiance curves for different painting conditions. Light measurements were taken under full-spectrum light through plastic that was either painted or not. (B) Light-sensitivity of TIM abundance. Top, experimental paradigm. Painted antennae were harvested at CT18 (arrows). Middle, blot of TIM levels from pooled antennae painted clear (C) or black (B) from butterflies in dark or light pulsed. Bottom, quantifications. Values are mean ± SEM of 3–4 antennae. White bars, clear-painted antennae; black bars, black-painted antennae. p-values, Student’s t-test: **, p < 0.005. (C) Temporal patterns of per and tim expression in brains (left column) and antenna (right column) from butterflies with the antennae painted clear or black. Values are mean ± SEM of 3 animals, except for the three points without error bars that represent the mean of 2 animals. Box shading: open, light; dark gray, night; and light gray, subjective day. p-values, one-way ANOVA.