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. 2009 Oct 7;4(10):e7329. doi: 10.1371/journal.pone.0007329

Figure 2. Relative retinal expression level of GFAP, Kir4.1, AQP4, Utrophin and β-dystroglycan after retinal detachment.

Figure 2

A: 24 h after surgery, proteins of control and detached retina from C57BL/6 mice were extracted and Western blotting was performed. Representative photograph of immunoblots reacted with anti-GFAP, anti-Kir4.1, anti-AQP4, anti utrophins, anti β-dystroglycan (β-DG) and β-Actin antibodies. Numbers on the left refer to the relative electrophoretic mobility of prestained molecular mass standards in kiloDaltons. B: The relative protein level is expressed in arbitrary units. Each value represents the ratio of the specific band stain intensity normalized to β-Actin expression (TotalLab TL120, Nonlinear Inc, Durham NC, USA). In detached retina, GFAP expression level was significantly upregulated while AQP4 expression was downregulated. There was no significant difference in Kir4.1, utrophin and β-DG protein expression level after retinal detachment. All experiments were repeated at least three times, and the bars represent means + SE for triplicate data points; n = 4. *p<0.05; ***p<0.001 significant differences versus control.