Table 1.

Solutions

Extracellular	NaCl	KCl	NaH2PO4	NaHCO3	Hepes	CsCl	TEA-Cl	CaCl2	MgCl2	Sucrose	Glucose	NMDG-Cl or TEA-CL or LiCl
Cutting	—	1	—	26	—	—	—	1	9	248	10	—
ACSF	125	2.5	1.25	25	—	—	—	2	2	—	10	—
ExTNM	26	—	—	—	10	10	112	0 or 0.5	4	—	11	—
ExTNM Na+ subst.	—	—	—	—	10	10	112	0.5	4	—	11	26
ExTNM + 2 K+	26	2	—	—	10	10	112	0.5	4	—	11	—
ExTNM Na+
subst. + 2 K+	—	2	—	—	10	10	112	0.5	4	—	11	26
Intracellular	Potassium gluconate			KCl	CsCl	Hepes	EGTA	MgCl2	Tris-GTP	Mg-ATP	Sodium phosphocr.
CsCl	—			—	133	10	10	2	0.4	4	10
Pot gluc	110			20	—	10	10	2	0.4	4	10
CsCl + KCl	—			20	113	10	10	2	0.4	4	10

Concentrations are indicated in mmol l⁻¹. In voltage clamp experiments, the following blockers were routinely added to ExTNM and ExTNM Na⁺ subst : TTX (1 μm), nifedipin (10 μm), mibefradil (10 μm). Flufenamic acid (10 μm) was added for n= 14 recordings as indicated. All intracellular solutions were added with 4 MgATP, 0.4 Tris-GTP and 10 Na₂-phosphocreatine (in mm). The pH of intracellular solutions was adjusted to 7.3 with KOH for the potassium gluconate-based solution and CsOH for the CsCl-based solution.