Fig. 1. Identification of mammalian Eme1. (A) Alignment of mouse, human and S.pombe Eme1 proteins with S.cerevisae Mms4. Amino acid identities and similarities are highlighted in black and gray, respectively. (B) Mouse Eme1 is mainly expressed in proliferative tissues. Radiolabeled Eme1 full-length cDNA was used to probe northern blots of mouse ES cells (left panel), mouse embryos at day 7, 11, 15 and 17 of gestation (middle panel) and mouse adult tissues (right panel). Northern blots were subsequently probed with a GADPH cDNA to assess loading.