Fig. 7A, B.

Localization of Cx36-like immunoreactivity. A, B Photomicrographs of double immunofluorescence staining of a 12-µm horizontal section from an E6 chicken. Rabbit polyclonal anti-Cx36 antibodies and Cy2-conjugated goat anti-rabbit IgG antibodies (green) were used to detect Cx36-like protein, and a mouse monoclonal anti-chicken sarcomeric myosin (MF20) antibody and Cy3-conjugated goat anti-mouse IgG antibodies (red) were employed to label myotome cells. Connexin36-like staining (arrows) is shown in A (green channel alone), and its relationship to sarcomeric myosin is demonstrated in B (superposition of green and red channels). Punctate Cx36-like immunoreactivity (arrows) was observed between cells of the myotome (M). The lack of an extensive superposition between the red and green signals is due to the different cellular localizations of the proteins: Cx36-like immunoreactivity localizes to the plasma membrane, whereas MF20 immunoreactivity is present in the cytoplasm