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. Author manuscript; available in PMC: 2009 Sep 29.
Published in final edited form as: J Mol Biol. 2007 Feb 22;368(2):537–549. doi: 10.1016/j.jmb.2007.02.040

Figure 3.

Figure 3

(A) Cleavage of 100 µM folded PEG-HNP1 by 25 mg/ml CNBr in 2.5% TFA. For comparison, 5 nmol of folded PEG-HNP1 dissolved in water was injected as control (thin line). An identical amount of protein was analyzed after one-hour cleavage by CNBr (thick line), showing a more than 75% conversion to HNP1. (B) Folded polyR-HNP1, polyS-HNP1 and crproHNP1 analyzed by RP-HPLC, prior to CNBr cleavage. (C) CNBr cleavage of polyR-HNP1, polyS-HNP1 and crproHNP1 monitored by RP-HPLC. The common cleavage product-HNP1 eluted at 15.5 min. Peaks eluted after 16 min were cleaved products derived form crpro peptide, whereas peaks eluted before HNP1 were cleaved products derived from polyR and polyS. The chromatographic data were collected under the same conditions as described above. The chromatographic data presented in this figure were collected under the same conditions as described in the legend of Figure 2.