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(A) The fluorescence spectra of 10 µM crproHNP1 in 10 mM phosphate buffer, pH 7.4, and of 10 µM crproHNP1 in 10 mM phosphate, 6 M GuHCl, pH 7.4. (B) The fluorescence spectra of 10 µM HNP2, 10 µM crpro peptide, and a mixture of both at 10 µM each in 10 mM phosphate buffer, pH 7.4. The spectroscopic data were collected at room temperature with an excitation wavelength of 295 nm, and averaged from multiple scans with a deviation of λmax of ± 1 nm.
