Table 1.
List of genes and RT-PCR primers used
| Gene | Sequence | Annealing Temperature (°C) | Product Size(bp) |
|---|---|---|---|
| Oct 4 | CGACCATCTGCCGCTTTGAG CCCCCTGTCCCCCATTCCTA |
57 | 572 |
| Nanog | CCTCCTCCATGGATCTGCTTATTCA CAGGTCTTCACCTGTTTGTAG |
57 | 262 |
| GAPDH | GGGCGCCTGGTCACCAGGGCTG GGGGCCATCCACAGTCTTCTG |
60 | 531 |
| βIIITubulin | CTTGGGGCCCTGGGCCTCCGA GGCTTCCTGCAGTGGTACACGGGCG |
60 | 174 |
| GATA4 | TCCAAACCAGAAAACGGAAG CTGTGCCCGTAGTGAGATGA |
60 | 187 |
| Brachury | ACCCAGTTCATAGCGGTGAC ATGAGGATTTGCAGGTGGAC |
60 | 216 |
| BMP2 | TGTATCGCAGGCACTCAGGTCAG AAGTCTGGTCACGGGGAAT |
60 | 328 |
| GATA2 | TGACTTCTCCTGCATGCACT AGCCGGCACCTGTTGTGCAA |
60 | 244 |
| HAND1 | TGCCTCAGAAAGAGAACCAG ATGGCAGGATGAACAAACAC |
60 | 274 |
| BMP4 | GTCCTGCTAGGAGGCGCGAG GTTCTCCAGATGTTCTTTCG |
60 | 339 |
| βhCG | GCTACTGCCCCACCATGACC ATGGACTCGAAGCGCACATC |
55 | 95 |
| GCM1 | CGGAAACGCTGTCCCAACT CCCTTCAGGCTCAATGAGACG |
57 | 235 |
| IGF2 | CAATGGGGAAGTCGATGCTG CTTGGCGAGCACGTGAC |
61 | 421 |
| FGFR1 | GGACTCTCCCATCACTCTGCAT CCCCTGTGCAATAGATGATGATC |
56 | 109 |