Table 1.
Plasmids and oligonucleotide primers used in this study
| Plasmids or primers | Phenotypes or sequencesa | Source or reference |
| Plasmids | ||
| pHT304 | AmprEmr, E. coli-B. thuringiensis shuttle cloning vector, 6528 bp | [39] |
| pGFPuv | Ampr, plasmid vector carrying a variant of gfp, 3336 bp | CLONTECH Lab, Inc. |
| pBMB3305 | AmprEmr, pHT304 derivative harbouring insecticidal gene cry3Aa with promoter Pcry3Aa, ~12.6 kb | Laboratory collection |
| pMB172 | Ampr, pTYB12 derivative harbouring wlacD, 8867 bp | [20] |
| pMB160 | AmprEmr, pHT304 derivative harbouring mba-gfp fusion gene, 8194 bp | This study |
| pMB161 | AmprEmr, pHT304 derivative harbouring mbp-gfp fusion gene, 8667 bp | This study |
| pMB162 | AmprEmr, pHT304 derivative harbouring mbg-gfp fusion gene, 8648 bp | This study |
| pMB163 | AmprEmr, pHT304 derivative harbouring promoter Pcry3Aa, mbg and gfp, 9365 bp | This study |
| pMB164 | AmprEmr, pHT304 derivative harbouring Pcry3Aa, mbgn and gfp, 8466 bp | This study |
| pMB165 | AmprErmr, pHT304 derivative harbouring Pcry3Aa, mbgc and gfp, 9002 bp | This study |
| pMB166 | AmprEmr, pHT304 derivative harbouring Pcry3Aa, lysM1 and gfp, 8276 bp | This study |
| pMB167 | AmprEmr, pHT304 derivative harbouring Pcry3Aa, lysM2 and gfp, 8294 bp | This study |
| pMB168 | AmprEmr, pHT304 derivative harbouring Pcry3Aa, (mbgn)2 and gfp, 8777 bp | This study |
| pMB169 | AmprEmr, pHT304 derivative harbouring Pcry3Aa, (mbgn)3 and gfp, 9098 bp | This study |
| pMB173 | AmprEmr, pHT304 derivative harbouring Pcry3Aa and wlacD, 8879 bp | This study |
| pMB174 | AmprEmr, pHT304 derivative harbouring Pcry3Aa, (mbgn) 2 and wlacD, 9521 bp | This study |
| Oligonucleotide primersb | ||
| BT101 | 5'-CGAGAATTCTGACAAGTGCGACACGTT-3' | |
| BT102 | 5'-TTCTTCTCCTTTACTCATACAGAAAATATGTTTACCG-3' | |
| BT103 | 5'-CGGTAAACATATTTTCTGTATGAGTAAAGGAGAAGAA-3' | |
| BT301 | 5'-CGTGAATTCCACTGTCAGTATAACACC-3' | |
| BT302 | 5'-TTCTTCTCCTTTACTCATCCTAACTAAATATGGCAG-3' | |
| BT303 | 5'-CTGCCATATTTAGTTAGGATGAGTAAAGGAGAAGAA-3' | |
| P1 | 5'-TTACCCGGGCTTCCCTTCTTTCACTTC-3' | |
| P2 | 5'-TTCTTCTCCTTTACTCATGCCCTTTTTCGTAATCGT-3' | |
| P3 | 5'-ACGATTACGAAAAAGGGCATGAGTAAAGGAGAAGAA-3' | |
| P4 | 5'-AAACTGCAGTTATTTGTAGAGCTCATCCATGC-3' | |
| P5 | 5'-ACGGGAATTCGGATTCAAAATAGCCCTG-3' | |
| P6 | 5'-CTGTCTAGACGGATTCATTTTTCTTCC-3' | |
| P7 | 5'-CCAAGTTCTAGAATGATTCAAATTGTAACGG-3' | |
| P8 | 5'-GCTAGATCTGCCCTTTTTCGTAATCGT-3' | |
| P9 | 5'-ACGAGATCTATGAGTAAAGGAGAAGAA-3' | |
| P10 | 5'-TCTAGAGATCTGATGGATTCTACAGCTCG-3' | |
| P11 | 5'-CTGTCTAGAGTTAATGCTACACGTGCC-3' | |
| P12 | 5'-GCAAGATCTAACGATAAGTGCCTGACC-3' | |
| P13 | 5'-CGCTCTAGATATGTACAGCCTGGTGAC-3' | |
| P14 | 5'-CGCGGATCCATGATTCAAATTGTAACGG-3' | |
| P15 | 5'-CTGTCTAGA ATGCAACGTCGTGATTTC-3' | |
| P16 | 5'-AAACTGCAGTTATACCGTAAACCCTAAC-3' | |
| P17 | 5'-GCAAGATCTATGCAACGTCGTGATTTC-3' |
a Emr, erythromycin resistance; Ampr, ampicillin resistance; cry3Aa, B. thuringiensis insecticidal gene;Pcry3Aa, the promoter of cry3Aa; gfp, green fluorescent protein gene; wlacD, a mutated S. dysenteriae laccase gene [20]; mbg, a putative N-acetylglucosaminidase gene from B. thuringiensis YBT-1520; mbgn, N-terminal domain of mbg; mbgc, C-terminal domain of mbg; (mbgn)2, two tandemly aligned repeats of N-terminal domain of mbg.
b The underlined sequences indicates the restriction enzyme sites.