Fig. 1. Targeting of BICD2 N–terminus to mitochondria and peroxisomes induces their relocalization. (A) Schematic representation of the fusion constructs of BICD2 or its domains to membrane targeting sequence (MTS) of ActA protein of Listeria monocytogenes. Coiled-coil regions of BICD2 are indicated with gray boxes. (B–S) HeLa cells were transfected with GFP-fusion constructs and stained for different cytoplasmic organelles. GFP signals are shown in the left panel, stained organelles in the middle panels, and an overlay of the portion of the figure, indicated by a white rectangle, (with GFP signal in green, organelles in red), in the right panels. (B–D) Cells were transfected with GFP-MTS and stained for mitochondria with MitoTracker Red CMXRos. (E–G) Cells were transfected with GFP-MTS and stained for a peroxisomal marker PEX1 [in (G) peroxisomes are indicated by arrows, and mitochondria by arrowheads]. (H–J) Cells were transfected with GFP-BICD2-MTS and stained for mitochondria. (K–S) Cells were transfected with GFP-BICD-N-MTS and stained for mitochondria (L), peroxisomes (O), and a centrosomal marker γ-tubulin (R). MTOCs are indicated by arrows in (R). Bar, 10 µm.