Fig. 7. Dynamitin inhibits aggregation of mitochondria and peroxisomes, caused by BICD2-MTS. (A–C) HeLa cells were cotransfected with GFP-BICD2-MTS and HA-dynamitin, stained with MitoTracker, fixed and stained for HA-tag. GFP signal is shown in (A), MitoTracker signal in (B) and HA-specific signal in (C). (D–F) HeLa cells were transfected with GFP-BICD2-MTS (D) and stained for peroxisomal marker PEX1 (E), overlay is shown in (F) (GFP signal in green, PEX1 signal in red). (G–I) HeLa cells were cotransfected with GFP-BICD2-MTS (G) and HA-dynamitin and stained for PEX1 (H) and HA-tag (I). Bar, 10 µm. (J) Quantitative analysis of the transfection results, shown in (D–I), performed as described for Figure 6P. Only cells which had a healthy, spread appearance and expressed medium levels (5–10 times compared with the endogenous BICD2 levels, as determined by counterstaining with anti-BICD2 antibodies), were scored. Staining for peroxisomes, rather than mitochondria, was used here for quantification, because it allows better assessment of the partially and fully dispersed phenotypes.