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. 2009 Oct 8;4(10):e7347. doi: 10.1371/journal.pone.0007347

Figure 3. RT-PCR analysis of gene expression for sour-ageusic Patient 8716.

Figure 3

The patient expresses transcripts for housekeeping genes and those involved in sweet, bitter and salty taste, but ASIC transcripts are undetectable even after 50 cycles of amplification. Lane identifications: (A) 100 bp DNA marker with the brightest band being 500 bp; (B) Transcript for 460-bp fragment of β-actin; (C–G) Transcripts for, respectively, ASICs 1a, 1β, 2a, 2b and 3; (H) Transcript for PLC-β2, which is involved in the transduction pathways of sweet and bitter; (I) 1 kb DNA marker; (J) full coding sequence of β-actin; (K) full coding sequence of bitter receptor T2R14; (L) full coding sequence of δ-ENaC; and (M) partial coding sequence of α-ENaC. RT-PCR was performed with cDNA from fungiform papillae but only the data for the tubes containing reverse transcriptase are presented. No products were detected in the tubes lacking reverse transcriptase. Individual amplification reactions were performed for each named molecular target and the data are shown in collated form. Identities of all amplification products were confirmed by sequencing.