Table 1. List of primers used in this study.
Target Transcript Or Gene | GenBank Accession Number | Primer Name | Orientation | Sequence 5′→3′ | Product Size (bp) |
ASIC1a | U78181 | BNACF11 | Forward | CAACAAGGATGGAACTGAAGGCCGA | |
BNACR12 | Reverse | ATCTAGGCCTTTGGTTCAGCGG | 1627 | ||
ASIC1a | U78181 | HASIC3 | Forward | GTACTGCGTGTGTGAAATGCC | |
HASIC4 | Reverse | TGTTGGCAGCGTATGTCATC | 461 | ||
ASIC1 | U78181 | HASIC3 | Forward | GTACTGCGTGTGTGAAATGCC | |
HASIC4 | Reverse | TGTTGGCAGCGTATGTCATC | 1535 | ||
ASIC1 | U78181 | ASICMCF1 | Forward | TGGCCCACATCTTCTCCTAC | |
ASICMCR1 | Reverse | CATCTGCCATCTGTGTGTCT | 936 | ||
ASIC1β | AJ006519 | ASICβF1 | Forward | ATGGAGGCAGGGTCGGAGTT | |
ASICβR1 | Reverse | GGCCCCACAGTAGGAACAA | 490 | ||
ASIC2a | U57352 | HBNAC1AF | Forward | ACAGGAGCAGAGGCTCACAT | |
HBNAC1DR | Reverse | TGAACAATCCCATCTGACCA | 500 | ||
ASIC2b | Y14635 | RASIC2BF2 | Forward | CACTAAATTGCACGGGCTG | |
RASIC2BR2 | Reverse | GCATATCCTCCAGCTGGTG | 467 | ||
ASIC3 | AF057711 | HDRASICF2 | Forward | TTCACCACGATCTTCACCCG | |
HDRASICR2 | Reverse | ACGTCGCCTGGCATGTACAC | 491 | ||
β-actin | NM_001101 | HBACTINF1 | Forward | ATGGATGATGATATCGCCGCGC | |
HBACTINR1 | Reverse | CTAGAAGCATTTGCGGTGGAC | 1128 | ||
β-actin | NM_001101 | HBACTINF2 | Forward | CGTGACATTAAGGAGAAGCT | |
HBACTINR2 | Reverse | CATACTCCTGCTTGCTGATC | 460 | ||
β-actin | NG_003162 | ACTINX4F. ACTINX5R | Forward, Reverse | TCATGTTTGAGACCTTCAA, GTCTTTGCGGATGTCCACG | 506 |
PKD1L3 | AY164485 | HPKD1F1 | Forward | GAACTCTGCTGCGACTCACC | |
HPKD1R1 | Reverse | TGTCACTGCCCACTGCTGTCGT | 530 | ||
PKD2L1 | NM_016112 | HPKD2F1 | Forward | ACACTGAGATTGAGAAACTAGGCCG | |
HPKD2R1 | Reverse | GCCTCACACTTAACTCCTCTGC | 410 | ||
PKD2L1 | NM_016112 | HPKD2F1 | Forward | ACACTGAGATTGAGAAACTAGGCCG | |
HPKD2R1 | Reverse | GCCTCACACTTAACTCCTCTGC | 2123 | ||
PLCβ2 | NM_004573 | PLCBF1 | Forward | AGGAGCAGTACGAGTGCGTT | |
PLCBR1 | Reverse | CTTCACCTCTGCCTCCAGAC | 431 | ||
T2R14 | AF227138 | BTR14F1 | Forward | ATGGGTGGTGTCATAAAGAG | |
BTR14R1 | Reverse | TCAAGATGATTCTCTAAATTCT | 954 | ||
δ-ENaC | U38254 | HENACDF1 | Forward | ATGGCTGAGCACCGAAGCATGGAC | |
HENACDR3 | Reverse | GAGGTTGACGTTGTACAGGGA | 501 | ||
δ-ENaC | U38254 | HENACDF1 | Forward | ATGGCTGAGCACCGAAGCATGGAC | |
HENACDR1 | Reverse | GGTGTCCAGAGTCTCAAGGGG | 1917 | ||
α-ENaC | L29007 | HENACAF1 | Forward | ATGGAGGGGAACAAGCTGGAGGAG | |
HENACAR1 | Reverse | GGAGCATCTGCCTTGGTGTGAG | 2048 |