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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: FASEB J. 2008 Feb 19;22(6):1886–1893. doi: 10.1096/fj.07-100925

Figure 4.

Figure 4

Xp-HB-IGF-1 is retained in cartilage tissue. A, B) Cartilage disks were incubated with 500 nM Xp-HB-IGF-1 or Xp-IGF-1 for 2 days, then washed 3× with PBS and changed to media with no IGF-1 (day 0). IGF-1 fusion proteins remaining in the cartilage were detected by Western blot analysis with an anti-IGF-1 antibody (A) or by an ELISA using anti-Xpress and anti-IGF-1 antibodies (B). Results are expressed as mean ± se; n = 4. *P < 0.01. NS, not significant. C) No treatment cartilage disks or chondroitinase-treated cartilage disks were incubated with 500 nM Xp-HB-IGF-1 or Xp-IGF-1 for 2 days, then washed 3× with PBS and changed to media with no IGF-1 (day 0). IGF-1 fusion proteins remaining in the cartilage were detected by Western blot analysis with an anti-IGF-1 antibody.