Table 3.
Conventional Microscopy |
Fluorescence Microscopy |
||||
---|---|---|---|---|---|
Smear Result* | Total | Culture-positive (N, %) | Total | Culture-positive (N, %) | p-value† |
Negative | 485 | 131 (27%) | 446 | 124 (28%) | 0.58 |
Scanty (1–9 AFB/100 fields) | 48 | 43 (90%) | 67 | 36 (54%) | <0.001 |
1+ (10–99 AFB/100 fields) | 70 | 64 (91%) | 57 | 47 (82%) | 0.02 |
2+ (1–10 AFB/field) | 41 | 40 (98%) | 45 | 43 (96%) | 0.62 |
3+ (> 10 AFB/field) | 72 | 72 (100%) | 101 | 100 (99%) | N/A†† |
Abbreviations: AFB, acid-fast bacilli
To compare conventional and fluorescence microscopy on the same scale, the number of AFB observed with fluorescence microscopy was divided by a correction factor of 10 to adjust for the lower level of magnification. The actual number of AFB observed with fluorescence microscopy was: Negative, 0 AFB/length; Scanty, 1–99 AFB/length; 1+, 100–999 AFB/length; 2+, 10–100 AFB/field; and 3+, > 100 AFB/field.
Compares proportion of positive cultures within each smear result category for conventional and fluorescence microscopy.
3+ smear result category dropped from the model due to co-linearity.