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. Author manuscript; available in PMC: 2010 Oct 6.
Published in final edited form as: Biochemistry. 2009 Oct 6;48(39):9250–9255. doi: 10.1021/bi9014067

Fig. 4.

Fig. 4

Galactose efflux by intact E. coli HB101. Intact E. coli 101 (lacZ+Y−) expressing wild type, mutant D240E or empty vector were adjusted to an OD420 of 10.0 (0.7 mg protein/ml). Cells were incubated with 0.4 mM lactose (light grey column) for 1 h. After filtration, the filtrates were assayed for galactose and lactose (light gray column) by nuclear magnetic resonance.