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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Cell Biochem. 2009 Oct 15;108(3):621–630. doi: 10.1002/jcb.22294

Fig. 3.

Fig. 3

Detection of NPY protein by immunofluorescence. A,B: DMP1-GFP expression in mouse calvarial osteoblast cell cultures at day 16 prior to cell sorting under epifluorescence (A) and phase contrast (B). C: Epifluorescent image of DMP1+ cells after sorting. D: Epifluorescent imaging shows no GFP expression in DMP1− cells following sorting. E: DMP1+ cells and (F) DMP1− cells immunostained for NPY (red, TRITC+), and nuclear staining (blue, DAPI). A strong immunoreactivity was observed in DMP1+ cells (E), while a lower but detectable expression was observed in DMP1− cells (F).