Skip to main content
. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: J Cell Biochem. 2009 Oct 15;108(3):621–630. doi: 10.1002/jcb.22294

Fig. 7.

Fig. 7

Effect of NPY on intracellular cAMP levels. A: Effect of treatment with NPY on the intracellular cAMP levels in primary mouse calvarial osteoblasts cultures. Cells were treated with 10nM NPY for 1 h while the control group received vehicle only. Values represent cAMP levels at days 7 and 14, as assessed by ELISA (n = 2). B,C: Effect of NPY on forskolin-induced intracellular cAMP levels primary calvarial osteoblasts cultures. D: The effect of NPY-Y1 blocker BIB03304 on the regulation of intracellular cAMP levels in primary calvarial osteoblast cultures. Cells were cultured for 7 days and then treated with forskolin alone or with either NPY or NPY-Y1 blocker BIB03304 (values are mean ± SD from three experiments).