Figure 3.

MAR1 overexpression results in hypersensitivity to antibiotics. A, Seeds were plated on kanamycin (10 mg/L; Kan10). After 14 d, two representative seedlings of each line were photographed. Phenotypes of three independent overexpression lines are shown (a, b, and c). All lines are in the Ler background. Ler wt, Ler wild type. B, Chlorophyll content (μg of chlorophyll per mg fresh weight [FW]) of MAR1 overexpression lines grown for 2 weeks on media containing gentamicin (70 mg/L). OE-A, OE-B, and OE-C are three independent MAR1 overexpression lines. Measurements represent the average chlorophyll content (±sd) of three separate batches of seedlings. C, Overexpression of MAR1 in mar1-1 background reverses the kanamycin resistance phenotype of mar1-1. Mutant mar1-1 plants were transformed with 35S∷MAR1, and seeds were plated on kanamycin (25 mg/L). After 14 d of growth, two representative seedlings were photographed. [See online article for color version of this figure.]