Figure 5.

MAR1-YFP localizes to chloroplasts in leaves of transformed plants. Plants were transformed with the C-terminal fusion construct 35S∷MAR1-YFP as described in “Materials and Methods.” Confocal single-slice images of the leaves of two individually transformed plants (A–F and G–L) were compared to an untransformed leaf (M–O). D to F and J to L are close-up images of A to C and G to I, respectively. Chlorophyll autofluorescence (A, D, G, J, and M), YFP fluorescence (B, E, H, K, and N), and a merge of the two channels (C, F, I, L, and O) are shown for each leaf section. Bars = 8 μm.