Figure 1.

Arabidopsis plants produce P. furiosus GFP-SOR and grow normally. A, Expression of GFP-SOR in 14-d-old transgenic Arabidopsis plants is shown by RT-PCR analysis using an internal GFP forward primer and a SOR-specific reverse primer to detect the fusion transcript (top panel). Primers specific for Arabidopsis actin were used for the loading control (bottom panel). B, Immunoblot analysis indicates that GFP-SOR is produced in transgenic Arabidopsis plants. Protein was detected with antibodies raised against P. furiosus SOR. The bottom panel is the amido black-stained polyvinylidene difluoride membrane showing the protein extracts from each line. An equal amount (25 μg of protein) of total soluble protein was used in each lane. The GFP-SOR protein (42-kD predicted molecular mass) and smaller proteolytic products are detected in all SOR transgenic lines. C, Plants grown under short-day conditions (8 h of light/16 h of dark) have no obvious phenotype. Leaf size (24.8 ± 1.7 mm for the wild type, 23.4 ± 0.8 mm for GFP, 24.8 ± 1.8 mm for SOR3, and 21.6 ± 1.5 mm for SOR9) and number (36.0 ± 1.7 for the wild type, 39.5 ± 1.4 for GFP, 41.0 ± 0.8 for SOR3, and 39.5 ± 2.5 for SOR9) of 56-d-old plants were not statistically different for any of the SOR lines. WT, Wild type. [See online article for color version of this figure.]