Figure 3.

A, Seeds from SOR transgenics have a higher percentage of germination after heat treatment. One hundred seeds per line were used for the seed germination assay. The data are means ± sd from two independent experiments. B, SOR seedlings also had increased acquired thermotolerance. Acquired thermotolerance was tested by the hypocotyl elongation assay. Briefly, 2.5-d-old etiolated dark-grown seedlings were either maintained at 22°C or treated at 38°C for 1.5 h followed by 45°C for 2 h. Seedlings were returned to 22°C for 2.5 d in the dark. Plates were photographed, and hypocotyl growth was measured using Adobe Photoshop and analyzed using Microsoft Excel. A total of 21 to 31 seedlings per line were used for the hypocotyl elongation assay. The data are means ± sd. WT, Wild type.