HSP70, BiP, and CRT are less abundant in response to heat stress in SOR plants. Total protein was isolated, and an equal amount of protein (20 μg) was separated by SDS-PAGE, immunoblotted, and visualized with antibodies specific for the proteins indicated (top panels). The bottom panel is an amido black-stained polyvinylidene difluoride membrane showing the protein extracts from each line. The data are representative of two biological replicates that gave similar results. WT, Wild type.