Figure 4.
Confirmation of tvinv disruption. A, Southern-blot analysis of T. virens wild-type strain (Gv29-8) and tvinv deletion transformants (Δtvinv2 and Δtvinv5). An autoradiograph of a DNA gel blot hybridized with [32P]dCTP-labeled probe is shown in Supplemental Figure S2A. Fifteen micrograms of genomic DNA was digested with HindIII and loaded in each lane. The expected molecular sizes for native and deletion events are indicated on the left. B, TvInv activity in wild-type and mutant cells collected from hydroponic systems. Inv activity was determined in protein extracts from T. virens Gv29-8 and Δtvinv strains coinoculated with maize plants in hydroponic systems. The bar depicts mean values ± sd of two independent experiments. Nd, Not detected.