Figure 6.

Germling development and Suc perception by Δtvinv mutants and wild-type strains. A, Germling development of Gv29-8, Δtvinv2, and Δtvinv5 strains after 12 h on VMS medium incubated in moist dark chambers at 27°C. B, Schematic depicting the experimental designs to assess the differential use of sugars by each strain. Spore suspensions for each strain were placed in the center of the plates. Different combinations of two paper discs (sugar 1 and sugar 2) imbibed with 3% (w/v) Suc or 1.5% (w/v) Glc were placed 3 cm away from the center. C, The sugars are expected to diffuse and generate a concentration gradient on both sides of the initial inoculum. Fungal growth was evaluated after 24 h of incubation at 27°C.