Comparative analysis of maize sequence contigs with rice and sorghum. The two sequenced rf1-associated BAC contigs are shown in the center; predicted genes are shown as red rectangles on the black sequence contig lines, with gene identification numbers found in red above. Comparative sequence analysis with rice is shown at the top, and shared sequence regions between maize and rice are shown as green connecting lines. Comparative sequence analysis with sorghum is shown at the bottom, and shared sequence regions between maize and sorghum are shown as blue connecting lines. Collinear regions are seen between maize chromosome 3, rice chromosome 1, and sorghum chromosome 3. Seven out of 14 predicted genes are found in collinear order and in orientation between maize and rice. Ten out of 14 predicted genes are found in collinear order and in orientation between maize and sorghum; three of these genes are found duplicated in a second location on sorghum chromosome 3. One nonpredicted gene region at the left end of rf1-C1 aligns to collinear regions in both rice and sorghum. This is probably a maize pseudogene.