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. 2009 Oct;151(2):528–540. doi: 10.1104/pp.109.142521

Figure 5.

Figure 5.

Total levels of volatile Se in bacteria cells treated with various Se compounds (A–D), bacteria growth (E), Se accumulation (F), and Se tolerance (G). A to D, GC-MS analysis of total levels of volatile Se in the head space of bacterial cultures supplemented with 200 μm Na2SeO4 (A), Na2SeO3 (B), SeMet (C), or SeMCys (D) in LB medium. VC, Vector-only control. E, Effect of various forms of Se compounds on bacteria growth. Bacteria strains were grown in LB medium without (C) or with 200 μm Na2SeO4 (SeO4), Na2SeO3 (SeO3), SeMet, or SeMCys at 37°C for 24 h. Bacteria growth was measured at OD600. F, Total Se accumulation. Bacteria were grown in LB medium without (C) or with 200 μm Na2SeO4 (SeO4), Na2SeO3 (SeO3), SeMet, or SeMCys at 37°C for 24 h. Cell were pelleted, washed extensively, and subjected to inductively coupled plasma analysis. G, Effects of BoCOQ5-2 expression on bacteria Se tolerance. Cell densities of bacteria grown in the absence (C) and presence of 100 μm Na2SeO4 (SeO4) or Na2SeO3 (SeO3) in minimal M9 medium were measured at OD600. Data are means of three repeats. Lowercase letters above the bars indicate significant differences (P < 0.05). FW, Fresh weight.