Figure 6.
Expression profile of BoCOQ5-2 in broccoli, and subcellular localization of BoCOQ5-2 protein in transgenic Arabidopsis. A, Real-time PCR determination of the relative expression levels of BoCOQ5-2 in various tissues of broccoli treated with or without 20 μm Na2SeO4. The expression of BoCOQ5-2 in mature leaf without Se treatment was set to 1. YL, Young leaf; ML, mature leaf; Rt, root; Ft, floret; Fr, flower. Data are means of three repeats with two biological replicates. B, Subcellular localization of BoCOQ5-2-GFP. GFP signal in leaf epidermal cells of Arabidopsis plants expressing the BoCOQ5-2-GFP fusion (left), red fluorescence from MitoTracker Red (middle), and overlay of both GFP and MitoTracker Red signals (right) are shown. These images represent multiple analyses of three independent transgenic lines. Bar = 10 mm.