Figure 7.

Analysis of BoCOQ5-2 transgene levels, volatile Se content, ubiquinone level, and Se tolerance in transgenic Arabidopsis. A, Semiquantitative reverse transcription-PCR assay of BoCOQ5-2 transcript level in independent transgenic Arabidopsis lines. Expression of ACTIN (BoAct) was used as an internal control. B, GC-MS analysis of DMSe contents in transgenic Arabidopsis exposed to 50 μm Na₂SeO₃ for 3 d. C, Relative ubiquinone levels in transgenic Arabidopsis. D, Root length of transgenic Arabidopsis seedlings grown for 12 d on MS medium supplied with or without Se as indicated. E, Total Se accumulation. Transgenic Arabidopsis was treated with 50 μm Na₂SeO₃ for 3 d. Data represent means (n = 10) ± sd. F, In situ detection of ROS by DAB and NBT staining of representative leaves after exposing the transgenic plant to 25 μm Na₂SeO₃. The brown and purple precipitations indicate the production of ROS. Data are means of three repeats. Lowercase letters above the bars indicate significant differences (P < 0.05). DW, Dry weight; FW fresh weight; L3 and L5, transgenic line 3 and line 5; VC, vector-only control; WT, wild type. [See online article for color version of this figure.]