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. 2009 Oct;151(2):905–924. doi: 10.1104/pp.109.145144

Figure 1.

Figure 1.

Characterization of pnp1-1 and pnp1-2 T-DNA insertion mutants. A, Diagram of the two T-DNA insertions in the PNP gene corresponding to the lines named pnp1-1 and pnp1-2 (SALK_013306 and SALK_070705, respectively). B, Total (T) or chloroplast (cp) proteins extracted from the wild type (WT) and the pnp1-1 mutant were analyzed for RNA binding by the UV cross-linking assay as described in “Materials and Methods.” The bottom band is an unknown RNA-binding protein whose signal is similar in the different samples. C, The wild type, pnp1-1, and pnp1-2 homozygous mutants were grown on soil for 21 d. Note that the 1-cm bar size is different for the mutants. D, cpRNA patterns in the pnp1-1 and pnp1-2 mutants. Total RNA was isolated from the wild type, pnp1-1, and pnp1-2 and analyzed by ethidium bromide staining to reveal 23S rRNA anomalies (top) or by gel blot using the chloroplast gene probes indicated at right. [See online article for color version of this figure.]