Protein sequence analysis of BU1. A and B, Alignment (A) and phylogenic tree (B) of HLH domains for BU1 (Os06g0226500), its three homologous proteins, PRE1, and two bHLH proteins associated with BR signaling, BEE1 and BIM1. The top five protein sequences are full length. HLH domains are indicated below the sequences. Black and gray backgrounds indicate identical and similar amino acids, respectively. The bar corresponds to 0.1 amino acid substitutions per site.