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. 2009 Oct;151(2):936–948. doi: 10.1104/pp.109.145623

Figure 5.

Figure 5.

OsWRKY13 protein binds to the promoters of OsWRKY45-1 and OsWRKY45-2. Chromatin immunoprecipitation assay was performed with the extracts from the leaves of Mudanjiang 8 and Minghui 63 seedlings. Real-time PCR was conducted before immunoprecipitation (input), after immunoprecipitation (IP) with anti-OsWRKY13 antibody (+), or after immunoprecipitation without anti-OsWRKY13 antibody (−). The amplification of the 3′ untranslated region of OsWRKY45-1 or OsWRKY45-2 using PCR primer pair w45F/R served as a sample quantity control. The amounts of target PCR products from IP are relative (IP/input %) to those from input. Bars represent means (three replicates) ± sd.