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Determination of mRNA levels of various cell-cycling genes by RT-PCR. Total RNAs were isolated from the first two leaves of wild-type Columbia (Col) and the gif1/2/3 triple mutant (gif) at day 6 and used for RT-PCR analysis. The numbers at the top indicate dilution factors of cDNAs derived from the RT reaction. ACTIN8 (ACT8) was used as an amplification control.
