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. 2009 Jul 9;150(10):4701–4712. doi: 10.1210/en.2009-0389

Figure 1.

Figure 1

cAMP regulation of CCND1 expression in endometrial stromal fibroblasts from women without vs. with endometriosis. A, Endometrial stromal fibroblasts from patients without (no endo, gray bars, n = 4) endometriosis were treated with vehicle (Ctrl) or 0.5 mm 8-bromo-cAMP (cAMP) for 4 d in 2% serum-containing medium, and CCND1 protein expression was analyzed by Western blot. B, Endometrial stromal fibroblasts from patients without (n = 6) or with (endo, white bars, n = 6) endometriosis were treated as above, and CCND1 mRNA expression was analyzed by quantitative RT-PCR. All values were normalized by RPL19 for total actin and mRNA expression for protein levels. C, Endometrial stromal fibroblasts from patients without (n = 5) or with (n = 4) endometriosis were treated as above, and cell proliferation was analyzed by BrdU incorporation. Bar graphs (least square means ± sem) represent fold changes relative to control (Ctrl, no endo, without cAMP). Means with asterisks and same letters indicate significant differences at P < 0.05 by Student’s t test and two-way ANOVA followed by Bonferroni post hoc test, respectively.