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. 2009 Jul 9;150(10):4701–4712. doi: 10.1210/en.2009-0389

Figure 2.

Figure 2

Inhibition of PKA by H89 in endometrial stromal fibroblasts from women without vs. with endometriosis. A and B, Endometrial stromal fibroblasts from patients without (no endo, gray bars, n = 6) or with (endo, white bars, n = 6) endometriosis were treated with DMSO vehicle (no inh), 1 μm H89, or 5 μm H89 for 30 min in 2% serum-containing medium, followed by addition of vehicle (Ctrl) or 0.5 mm cAMP for 4 d. Treatments and medium were refreshed every 2 d. CCND1 (A) and IGFBP1 (B) mRNA expression were analyzed by quantitative RT-PCR. All values were normalized by RPL19. Bar graphs (least square means ± sem) represent fold changes relative to control (no inh/no endo). Means with same letters indicate significant differences within treatments at P < 0.05 using two-way ANOVA by ranks, followed by Bonferroni post hoc test. Means with asterisks indicate significant differences in endo vs. corresponding no endo treatment at P < 0.05 using Mann-Whitney U test.