Figure 5.

Immunohistochemistry for ERK phosphorylation in eutopic endometrium of women with or without endometriosis. A, Representative photomicrographs of immunostaining for Tyr₂₀₄-phosphorylated ERK (pERK) (brown) in paraffin sections of human endometrium from women without (no endo, white bars) vs. with (endo, gray bars) endometriosis during different phases of the menstrual cycle. Panels are shown at ×200 magnification. B and C, Graphical representation of pERK staining in stromal cells (B) and epithelial cells (C) is shown as least square means ± sem and presented as percent pERK-positive cells. Means with same letters indicate significant differences across the menstrual cycle at P < 0.05 using Kruskal-Wallis ANOVA, followed by Dunn’s multiple comparison test. Means with asterisks indicate significant differences in endo vs. corresponding no endo treatment at P < 0.05 using Mann-Whitney U test. For PE phase, no endo n = 3 and endo n = 5; for ES, no endo n = 5 and endo n = 6; for MS, no endo n = 3 and endo n = 4); for LS, no endo n = 4. EP, Epithelial cells; ST, stromal cells.