Figure 5.

Effects of MXC exposure on the Dnmt expression pattern in adult ovaries. Ovaries from females that were treated with vehicle (control), 20 μg/kg·d MXC, or 100 mg/kg·d MXC between E19 and PND7 were collected on PND 50–60. RNA isolation and semiquantitative RT-PCR were performed as described in Materials and Methods. Housekeeping gene Hprt was used as an internal control. A representative gel is shown (A). Quantification (B) showed a significant stimulation of Dnmt3b by MXC treatment. *, P < 0.05 (n = 3–4 ovaries/treatment), and all PCRs were repeated three times. The error bars are not apparent in two groups in Dnmt3b graph due to small variation. The multiple bands in the Dnmt3a and 3b represent the different isoforms of each Dnmt.