Figure 2.

A, Inhibition of the ERK1/2 MAPK pathway diminishes the CRF-induced Akt phosphorylation. THP-1 cells were stimulated with CRF (10⁻⁷ m) alone or cochallenged with PD98059 (30 μm) for 30 min. Akt phosphorylation was examined by Western blot analysis. A representative Western blot of the phosphorylated (P-Akt) and total Akt is presented on the top, together with a graphical representation of the combined data of four individual experiments. Phosphorylated Akt expression was standardized to total Akt and represented as percentage of the control. *, P < 0.05 relative to control. B, Inhibition of the NF-κB pathway abolishes the CRF-induced Akt phosphorylation. THP-1 cells were stimulated with CRF (10⁻⁷ m) alone or cochallenged with PDTC (100 μm) for 30 min. Akt phosphorylation was examined by Western blot analysis. A representative Western blot of the phosphorylated and total Akt is presented on the top, together with a graphical representation of the combined data of four individual experiments. Phosphorylated Akt expression was standardized to total Akt and represented as percentage of the control. *, P < 0.05 relative to control.